Coding

Part:BBa_K2557035:Design

Designed by: Sheng XU   Group: iGEM18_NAU-CHINA   (2018-10-09)


TagRFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 73
    Illegal SapI site found at 690


Design Notes

In order to facilitate cloning, we will add a few amino acids to the front end in actual use.


Source

We cloned it from a plasmid borrowed from other laboratories in the school.

References